Temmel, Nyssa A. .
Identifying the genes involved in gender differentiation in Populus trichocarpa.
WHILE the majority of angiosperm plants have hermaphroditic flowers, approximately 6% of these species are dioecious. This sexual system has been described from a morphological and physiological stand point, though not much is known about how gender is determined at the molecular level, or how dioecy has evolved. The recently sequenced genome of the dioecious tree Populus trichocarpa provides a unique tool for studying the evolution of gender dimorphism. Because P. trichocarpa does not have sex chromosomes, I am using a microarray approach to identify genes that are differentially expressed between males and females, and then place these genes on the physical context of the genome, and study specific sets of candidate genes in order to map the genomic architecture of gender. Thus far I have established that there is a gender biased expression pattern in both leaf and floral tissue between males and females, and I am using RT-PCR to confirm these gender-biased expression patterns of selected genes in male and female leaf tissue for a larger number of individuals.
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UBC Botanical Gardens and Centre for Plant Reserach Website
UBC Department of Botany
1 - University of British Columbia, Botany Department, 3529-6270 University Boulevard, Vancouver, British Columbia, V6T 1Z4, Canada
Presentation Type: Poster:Posters for Sections
Location: Auditorium/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 12:30 PM