Barsalobres-Cavallari, C.F. , Maluf, M.P. , Maia, I.G. .
Real-Time PCR analysis of fruit-specific genes from Coffea arabica.
THE quality of a coffee beverage is directly related to the biochemical composition and structural features of the mature grain. However, little is known concerning the influence of important grain components such as polysaccharides, proteins and lipids, on coffee quality. One approach to address this question is to study the expression of genes encoding these major components, or enzymes involved in their synthesis. Towards this goal, this work describes the isolation and characterization of cDNAs encoding a group of proteins that are specifically expressed during grain development. Candidate ESTs encoding such products were mined using the Brazilian Coffee EST database and their fruit specific expression validated by RT-PCR. By using a constitutively expressed “housekeeping” gene encoding GAPDH as a reference gene to normalize mRNA levels, and by taking data from the exponential phase of the PCR amplification, we obtained a reliable measurement of the relative abundance of 8 fruit-specific genes from Coffea arabica. These expression profiling data provide a useful tool for promoter characterization in coffee and other economically important grains.
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1 - UNESP - Campus de Botucatu, Departamento de Genética, Rubião Júnior s/n, Botucatu, São Paulo, 18618-000, Brazil
2 - Embrapa - IAC, Centro de Café Alcides Carvalho, Av. Theodureto de Almeida Camargo, 1500, Campinas, São Paulo, 13001-970, Brazil
Presentation Type: Poster:Posters for Sections
Location: Auditorium/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 12:30 PM