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Abstract Detail


Systematics Section / ASPT

McCoy, Skip K R [1], Kuehl, Jennifer V. [2], Boore, Jeffrey L. [2], Raubeson, Linda A. [1].

The complete plastid genome sequence of Welwitschia mirabilis.

THE Gnetales are an enigmatic group of plants whose placement within the seed plant phylogeny has been problematic, partly due to a lack of data. Here we report on the completely sequenced plastid genome of Welwitschia mirabilis (WEMI), the first from a gnetalean. Draft sequence was generated from an RCA template amplified from isolated chloroplasts, the quality of the draft sequence was confirmed, and the Single Copy-IR boundaries were determined by PCR amplification and sequencing. The completed genome was annotated using Dual Organellar GenoMe Annotator (DOGMA). The WEMI genome is 119,724 base pairs (bp) long, with an Inverted Repeat (IR) of 20,006 bp, a Large Single Copy (LSC) of 68,556 bp, and a Small Single Copy (SSC) of 11,156 bp. The LSC end of the WEMI IR corresponds to the 495 bp remnant “IR” described in Pinus thunbergi. We have detected several anomalies in the WEMI genome, including the duplication of the trnR-CCG gene, the partial duplication of the trnF-GGA gene, and the loss of the petD intron. Plus, there are changes in gene order in both the LSC and SSC regions. There are also a number of genes absent (or present only as pseudo-genes), including all ndh genes, another feature shared with Pinus.


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1 - Central Washington State University, Department of Biological Sciences, Ellensburg, Washington, 98926-7537, USA
2 - DOE Joint Genome Institute, Department of Evolutionary Genomics, 2800 Mitchell Drive, Walnut Creek, California, 94598, USA

Keywords:
Gnetales
chloroplast genome
Gne-pine hypothesis.

Presentation Type: Poster:Posters for Sections
Session: 48-142
Location: Auditorium/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 12:30 PM
Abstract ID:222


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