Unable to connect to database - 10:06:27 Unable to connect to database - 10:06:27 SQL Statement is null or not a SELECT - 10:06:27 SQL Statement is null or not a DELETE - 10:06:27 Botany 2006 - Abstract Search
Unable to connect to database - 10:06:27 Unable to connect to database - 10:06:27 SQL Statement is null or not a SELECT - 10:06:27

Abstract Detail


Evolutionary Developmental Biology (Evo-Devo)

Yoo, Mi-Jeong [1], Koh, Jin [1], Soltis, Pamela S. [2], Soltis, Douglas E. [1].

Analysis of expression patterns of MADS-box gene homologues in Nymphaea odorata (Nymphaeaceae).

NYMPHAEA odorata Ait (Nymphaeaceae) possesses an undifferentiated outer and inner perianth and exhibits a gradual transformation from petaloid staminodes to functional stamens. To provide insights into the developmental genetics of the indistinct outer and inner perianth and the transformation series of staminodes in N. odorata, we examined the expression patterns of homologues of A, B, C, and E-function MADS-box genes. We isolated and characterized seven MADS-box gene homologues from N. odorata. Phylogenetic analyses revealed that they belong to the SQUA, DEF, GLO, SEP, and AGL6 gene lineages, and we refer to them as Nyod.AP1, Nyod.AP3, Nyod.PI, Nyod.AG1, Nyod.AG2, Nyod.SEP1, and Nyod.AGL6. The expression patterns of these genes in different floral organs were investigated using relative quantitative reverse transcription PCR (RQ-RT-PCR). At early developmental stages, Nyod.PI, Nyod.AP3, and Nyod.AG2 transcripts accumulated in all floral parts, but Nyod.AG1 transcripts were present only in staminodes, stamens, and carpels. Later in development, Nyod.PI and Nyod.AP3 transcripts were restricted to perianth, staminodes, and stamens, and Nyod.AG2 and Nyod.AG1 transcripts were limited to inner perianth, staminodes, and stamens. Nyod.AGL6 transcripts accumulated in perianth, staminodes, and ovules at early developmental stages, but were restricted to perianth and staminodes at later stages. Nyod.SEP1 transcripts accumulated in all floral parts regardless of developmental stage. Expression of transcripts was also examined using in situ hybridization for several genes, and the results are comparable to those obtained with RQ-RT-PCR. The expression patterns of Nyod.AP3, Nyod.PI, and Nyod.SEP1 in early stages are quite similar to those reported from Nuphar advena. However, Nyod.AG1, Nyod.AG2 and Nyod.AGL6 genes show different expression patterns from those obtained from Nuphar advena. In Nymphaea, as in other basal angiosperms, floral organ identity genes generally show broader patterns of expression than do their homologues in eudicot model organisms.


Log in to add this item to your schedule

1 - University of Florida, Department of Botany, 220 Bartram Hall, P.O. Box 118526, Gainesville, Florida, 32611-8526, USA
2 - University of Florida, Department of Botany, Florida Museum of Natural History, P.O. Box 117800, Gainesville, Florida, 32611-7800, USA

Keywords:
Nymphaea odorata
MADS-box gene
relative-quantitative RT-PCR
in situ hybridization
basal angiosperms.

Presentation Type: Oral Paper:Papers for Topics
Session: 52-2
Location: 303/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 2:15 PM
Abstract ID:239


Copyright 2000-2006, Botanical Society of America. All rights