Tropical Biology Section
Mishra, Biswapriya Biswavasa .
Arabinogalactan proteins as markers of differentiation in East Indian Sandalwood tree.
THE East Indian Sandalwood tree, i.e., Santalum album L., known for its precious oil, superior quality heartwood, medicinal properties, and aesthetic values, was probed at various develomental phases, undergoing xylogenesis and differentiation. In vitro maintained callus, somatic embryo, and seedling at various stages were studied with respect to development of vasculature and arabinogalactan protein (AGP) content. Total extractable AGPs were quantified using the beta-glucosyl Yariv reagent by single-radial diffusion assay where gum arabica was the standard AGP source. AGP types were identified by running vertical SDS-PAGE after staining with beta-glucosyl Yariv reagent. Histochemical analysis was done after hand-sectioning and proper fixation with Maule and Weisner reagents specific for detection of the lignin monomers and the double-staining of safranine and fast green for study of the detailed vasculature. From the study, it was seen that AGP could serve as a potential marker for differentiation in sandalwood, along with the histochemical analysis. The types of AGPs found were also stage specific and could serve as an indicator for identification of stage of develoment. -DU
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1 - Indian Institute of Technology(IIT), Biotechnology, c/o: Prof.Satyahari Dey, Professor and Head ,Plant Biotechnology Laboratory, Kharagpur, West Bengal, 721302, India
Presentation Type: Oral Paper:Papers for Sections
Date: Monday, July 31st, 2006
Time: 11:30 AM