Systematics Section / ASPT
Archibald, Jenny K. , Crawford, Daniel J. , Santos-Guerra, Arnoldo , Mort, Mark E. .
The utility of automated analysis of inter-simple sequence repeat (ISSR) loci for resolving relationships in the Canary Island species of Tolpis (Asteraceae).
PLANTS of oceanic islands are useful models for studying plant evolution and speciation because evolution is telescoped in time and space on oceanic archipelagos. Prior studies revealed little DNA sequence variation within the clade of ca. 10 Canary Island species of the genus Tolpis (Asteraceae – Cichorieae), which precluded resolving relationships among the species. The present study assessed the utility of automated analysis of inter-simple sequence repeat (ISSR) loci for resolving relationships within the clade, using both similarity (Dice coefficient) and Fitch parsimony methods. A total of 264 individuals from 36 populations of all recognized species and several undescribed morphological variants was included. Morphologically-distinct endemic species were resolved as distinct clusters in both types of analysis, while individuals from a large, morphologically-variable complex consisting primarily of T. laciniata and T. lagopoda were not well resolved as distinct at either the population or species levels. The neighbor-joining and parsimony strict consensus trees were not congruent at deeper levels such as the grouping of more than two populations or showing relationships among species.
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1 - Indiana University of Pennsylvania, Department of Biology, 975 Oakland Ave., Indiana, Pennsylvania, 15705, USA
2 - University of Kansas, Department of Ecology & Evolutionary Biology, Natural History Museum and Biodiversity Research Center, 1200 Sunnyside Avenue, Lawrence, Kansas, 66045-7534, USA
3 - Jardín de Aclimatación de La Orotava, Puerto de la Cruz, Canary Islands, Tenerife, , Spain
Presentation Type: Oral Paper:Papers for Sections
Date: Wednesday, August 2nd, 2006
Time: 3:30 PM