Molecular Ecology and Evolution
James, Karen , Schneider, Harald , Ansell, Stephen , Vogel, Johannes C. , Pedersen, Niklas , Newton, Angela , Robba, Lavinia , Evers, Margaret , Kilian, Andrzej .
Adapting Diversity Arrays Technology (DArT) for inter- and intraspecific molecular research in wild plants.
CONVENTIONAL techniques in molecular systematics typically involve time-consuming labor components that, while not prohibitive, limit the rate of scientific progress and pose a special challenge to large scale efforts. Initially developed for genetic analysis and breeding of crops, Diversity Arrays Technology (DArT) rapidly identifies and genotypes hundreds of DNA polymorphisms that differentiate closely related organisms. Because DArT does not require prior sequence information, it is suitable for non-model organism research, potentially bridging the gap between genome-era biotechnology and studies of the natural world. To begin exploring DArT as a tool for detecting and typing DNA variation within and among wild species, Diversity Arrays Technology Pty Ltd produced DArT datasets from genomic DNA extracted at the Natural History Museum from two small test groups, one selected from the fern Asplenium viride and close relatives, and the other from the moss Garovaglia elegans. Here we report the results of our characterization of the resulting DArT data, which allow us to infer the evolution of substrate specificity and the genomic composition of hybrids. Moreover, based on our results, we recommend specific methodological and analytical procedures for optimizing the DArT protocol for use in wild plant species. Lastly, we discuss the potential applications of DArT in different arenas of plant research and propose a strategy for using DArT not only for molecular plant systematics, population genetics, and evolutionary sciences, but also as a diagnostic tool for assigning unknown specimens to species and populations (i.e., as a complement to “DNA barcoding”) and inferring traits in plant evolution including identification of genomic regions correlated with particular adaptations.[c.e.:srb]
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1 - Natural History Museum, Department of Botany, Cromwell Road, London, SW7 5BD, England
2 - University of Goettingen, Department of Systematic Botany, Albrecht von Haller Institute for Plant Sciences, Untere Karspuele 2, Goettingen, D-37073, Germany
3 - Diversity Arrays Technology Pty Ltd, Building 1 CSIRO Forestry, Yarralumla, Canberra, ACT2600, Australia
Presentation Type: Oral Paper:Papers for Topics
Date: Monday, July 31st, 2006
Time: 4:45 PM