Evolutionary Developmental Biology (Evo-Devo)
Litt, Amy , Pan, Irvin , McElhinny, Elaine , McQuinn, Ryan , Giovannoni, James J. , Irish, Vivian F. .
Comparative morphological and transcriptional analysis of fruit development in tomato and flowering tobacco (N. sylvestris).
WE have initiated a combined morphological and transcriptional analysis of fruit development in Solanaceae aimed at identifying genes common to fruit development across Solanaceae as well as genes that play divergent roles in the development of dry and fleshy fruits. We have begun with a description of the morphological development of the Nicotiana sylvestris capsule, from anthesis through to dehiscence, and have identified benchmark stages comparable to those identified in post-fertilization tomato development for molecular analyses. We are also analyzing morphological development in Petunia, which, along with Nicotiana, has a plesiomorphic capsule, as well as species with an independent origin of a berry (Cestrum) and a reversal to a capsule (Datura). At the same time we are performing comparative transcriptome analyses of fruit development in these species, using the 12,000 unigene TOM2 microarray. Our initial experiments with 2 dpa fruits of tomato and N. sylvestris demonstrate that this technique is effective in detecting a large number of genes that are expressed at similar levels in both species, as well as significant numbers of genes expressed differentially in tomato or flowering tobacco. Preliminary analyses of transcripts whose expression is correlated with particular fruit types or are species specific will be presented.[c.e.:srb]
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1 - New York Botanical Garden, Institute of Systematic Botany, 200Th Street & Southern Boulevard, Bronx, New York, 10458-5126, USA
2 - Yale University, Molecular, Cellular, and Developmental Biology, P.O. Box 208104, New Haven, Connecticut, 06520, USA
3 - Cornell University, Boyce Thompson Institute, Ithaca, New York, 14853, USA
comparative fruit development
comparative transcriptome analysis.
Presentation Type: Oral Paper:Papers for Topics
Location: 303/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 3:45 PM