Gilding, Edward , Marks, M. David , Sanders, Mark .
Use of fluorescent proteins to monitor the interaction of transcription factors in Arabidopsis.
FLUORESCENT protein fusions have become vital utilities for use by geneticists and molecular biologists working in plant systems. Within recent years various forms of fluorescent proteins derived from cnidarians have been developed with a range of excitation and emission spectra. Here we describe the fusion of dsRed and GFP to Myb- and Myc-domain proteins, GL1 and GL3 respectively, which are involved in trichome development. According to yeast two-hybrid assays, GL1 and GL3 interact with each other along with at least one other factor to activate the expression of genes involved in trichome morphogenesis. The aim of this work is to determine if these two fluorescent proteins may act as a Fluorescent Energy Resonance Transfer (FRET) pair in plant cells as well as to determine if this protein-protein interaction is restricted to developing trichome cells. In addition to the use of FRET to visualize protein interactions in plant cells between two partners, Multicolor Bimolecular Fluorescent Complementation (MBiFC) constructs have been made and are in the process of being transferred to Arabidopsis. It is hoped that with the development of these tools in plants researchers will have the ability to visualize a wide range of protein-protein interactions as well as competition between interaction partners in vivo.
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1 - University of Minnesota, Plant Biology, 250 Biological Sciences Center, 1445 Gortner Ave, St. Paul, Minnesota, 55108, USA
2 - University of Minnesota, CBS Imaging Center, 123 Snyder Hall, 1475 Gortner Ave, St. Paul, Minnesota, 55108, USA
Presentation Type: Poster:Posters for Sections
Location: Auditorium/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 12:30 PM