Snow, Michael , Kistner, Erica , Renquist, Brett , VanSooy, Michael , VanSooy, Sean , Bard, Raymond .
A simple aeroponic chamber for the study of root exudates.
PAST studies have shown that roots of conifers produce a variety of secondary metabolites including monoterpenes and sesquiterpenes. Isolation and analysis of root exudates is complicated by possible contamination by soil, or dilution in hydroponic media. To overcome these difficulties a simple aeroponic growth system was constructed. Using off-the-shelf materials, the chamber was constructed from a pair of polyethylene canisters standing 0.6 m high with a total volume of 28 L. Roots are suspended in the chamber and sprayed with a half-strength Hoagland solution using a commercial mist nozzle driven by a pump. A commercially available cycle timer controls both the duration of mist application as well as the time interval between applications. The design allows for easy collection of samples of aqueous exudates using drippings from roots, or gaseous emissions using sampling ports. This apparatus was used to grow juvenile Douglas-fir trees for up to four months. Normal growth was established, including the development of secondary rootlets and needle flush. Periodic samplings of exudates were made and analyzed using capillary gas chromatography.
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1 - Universtiy of Portland, Biology, 5000 N Willamette Blvd., Portland, Oregon, 97203, USA
2 - Universtiy of Portland, Chemistry & Physics, 5000 N Willamette Blvd., Portland, Oregon, 97203, USA
Presentation Type: Poster:Posters for Sections
Location: Auditorium/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 12:30 PM