Developmental and Structural Section
Oney, Matthew , Bingham, Robin , Gravis, Curtis , Stark, Patrick .
Effects of simulated herbivory on tomato (Lycopersicon esculentum) leaf trichomes.
PREVIOUS work has shown that a variety of responses can be induced in some plants including trichome production and the production of secondary compounds. Extensive research has demonstrated chemical induction in the cultivated tomato. However, few studies have investigated whether trichome production is an inducible response in this species. Using the cultivated tomato (Lycospersicon esculentum), we compared the density of trichomes found on leaves after the application of four treatments. These treatments included: control, clip cage control, simulated herbivory, natural herbivory, and jasmonic acid. We grew twenty-five plants and randomly assigned five plants to each of the above treatments. Treatments were applied to the fourth true leaf and placed in the greenhouse in a randomized block design to control for spatial effects. We collected samples from the first new, fully expanded leaf after applying treatments and examined the tissue using scanning electron microscopy. We compared the density of trichomes on the terminal leaflet and the most proximal leaflet on each leaf. Our results showed a higher density of trichomes on the fifth leaf of plants that were treated with herbivory and jasmonic acid. We also found a significantly higher density of trichomes on the proximal leaflet than the terminal leaflet. There was no effect of treatment on the type of trichomes produced.
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1 - Western State College, Natural and Environmental Sciences, Gunnison, Colorado, 81231
2 - Western State College, Behavioral and Social Sciences, Gunnnison, Colorado, 81231, USA
Presentation Type: Oral Paper:Papers for Sections
Location: 312/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 11:15 AM