Developmental and Structural Section
Landrum, Vic , Alderfer, Ashley .
Effects of light level on wide-band tracheid initiation and development in leaf tissue cultures of Anacampseros rufescens (Portulacaceae).
WIDE-BAND tracheids (WBTs) are novel tracheids producing a wide secondary wall; hypothetically, the wide secondary wall prevents the primary wall from collapse and hydrogen-bonding during water stress. Earlier experiments have shown, with varying results, that light level plays a role in the initiation and development of WBTs from germination to flowering. To determine the effect of light levels, leaf tissue cultures of Anacampseros rufescens (Portulacaceae) were grown on callus initiation medium, allowed to multiply, and exposed to varying light levels as the independent variable. Light levels were no light, 2050, 6850, 7700, and 11 020 lux. Previous experiments had shown that WBT development was limited when light levels were below 4000 lux. Our hypothesis was that WBT numbers would increase with increasing lux level. Results show that only the 11 020 lux treatment showed WBT development. These results support the general hypothesis that higher light levels should initiate WBT formation. -DU
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1 - Washburn University, Biology, 1700 SW College Ave., Topeka, Kansas, 66621, USA
Presentation Type: Poster:Posters for Sections
Location: Auditorium/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 12:30 PM