| Abstract Detail
Mycological Section Panes, Vivian [1], Marayag, Patrick Joseph [1], Lao, Lawrence Edenino [1], Dalman, Ryan Em [1], Tolentino, Vivian [1], Villareal, Myra [1], Monotilla, Wilberto [1]. Isolation of Total RNA from the Medicinal Fungi Ganoderma lucidum Thom and Penicillium chrysogenum Curt Using The Modified Guanidine Method: A Comparative Approach. GANODERMA ludicum Curt and Penicillium chrysogenum Thom are fungi that have several medicinal properties. G. lucidum is used for the cure of various illnesses such as arthritis, insomnia and asthma. On the other hand, Penicillium chrysogenum is the source of the first antibiotic, penicillin. It acts against Gram-positive bacteria by disrupting its cell wall synthesis. This study aimed to isolate the total RNA from the said fungal species by modifying the Guanidium Thiocyanate-Phenol-Chloroform RNA Extraction Method. Some modifications and optimization to the procedure were introduced during the culture of fungal tissues, harvesting of samples, and in the actual isolation process. Liquid media was used in the pre-isolation procedure instead of the solid agar media suggested in the protocol and the alteration of the amount of the tissues from 50mg to 300mg was also employed. The use of a vacuum filtration set-up for harvesting was also employed. Modifications in the isolation protocol also included incubation in ice for 5 minutes after homogenization and changes in the amounts of reagents used such as adding 100ul more of denaturing solution to the tissues. Based on multiple analysis of variance, it was observed that the values for total RNA concentration were significantly different at P=0.05 level of significance but the values for RNA purity were not significantly different. The mean concentration of G. lucidum total RNA (17.7560 ug/ml) exceeded the mean concentration of P. chrysogenum (10.9760 ug/ml) by 6.78 ug/ml. A correlation between concentration and purity showed that the modified procedure is best suitable for G. lucidum in this study.
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1 - Ateneo De Manila University, Biology Department , School of Science and Engineering, Loyola Heights, Quezon City, 1108, The Philippines
Keywords:
fungi
total RNA
RNA
Isolation
protocol
modifications.
Presentation Type: Oral Paper:Papers for Sections
Session: 59-2
Location: 268/Holt
Date: Tuesday, August 1st, 2006
Time: 2:30 PM
Abstract ID:711 |
