Systematics Section / ASPT
Sass, Chodon , Stevenson, Dennis Wm. , Specht, Chelsea .
Optimizing the Barcode of Life: Preliminary data for Cycads.
RECENTLY, an effort has been undertaken to standardize a system of molecular markers that can be used to easily identify all named organisms. These molecular barcodes will enable the identification of organisms regardless of life stage and aid in the proper identification of ecological samples, partial material, gut contents, and disease vectors, among other applications. This tool could also be used in the field or at customs stations to prevent the trading of CITES listed species, such as members of the Cycadales. In
order to develop a functional barcode of life, we must identify genes that provide enough genetic diversity to delineate taxa while at the same time have enough similarity to allow the use of universal primers and amplification conditions. Plants are particularly challenging in this respect, however recently several sets of loci have been proposed for use in establishing a barcode for plants. One set of loci includes a nuclear region, the ITS
locus, and a chloroplast region, the psbA-trnH intergenic spacer. A second proposal suggests using five chloroplast gene regions – MatK, rpoC1, rpoB, accD and YCF5, with ndhJ as a potential sixth region. Data are presented from a study testing the ability of the two proposed gene sets to identify and distinguish among genera and species of cycads. The loci were tested for their ability to distinguish among the 10 described genera of cycads as well as to identify 43 out of 62 described species of Encephalartos and 7 out of 11 described species of Dioon. Identification to both generic and specific levels were carried out using algorithms based on alignment and percent identity, or a combination of several methods. An optimal algorithm and series of gene loci for use in identifying cycad taxa based on barcoding sequences will be suggested based on these comparisons.
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Consortium for the Barcoding of life
Royal Botanic Gardens, Kew
1 - University of California, Berkeley, Plant and Microbial Biology, 431 Koshland Hall, Berkeley, California, 94720, USA
2 - New York Botanical Garden, Institute of Systematic Botany, 200Th Street & Southern Boulevard, Bronx, New York, 10458-5126, USA
3 - University of California, Berkeley, Department of Integrative Biology, University and Jepson Herbaria, 1001 Valley Life Sciences Bldg. #2465, Berkeley, California, 94720, USA
Barcode of Life
Presentation Type: Poster:Posters for Sections
Location: Auditorium/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 12:30 PM