Systematics Section / ASPT
Lewis, Carl , Namoff, Sandra .
DNA barcoding for identification of palms: Sequencing is not the best strategy.
DNA barcoding is a promising approach for identifying plants that cannot be distinguished on the basis of morphology. For example, the living palm collection at Fairchild Tropical Botanic Garden includes juveniles that are decades away from developing reproductive structures that are diagnostic at the species level. We evaluated several DNA regions as potential sources of barcoding data for palms. Noncoding plastid regions and introns of protein coding nuclear genes were not variable enough for species identification. Nuclear ribosomal DNA exhibited intragenomic variation that obscured any diagnostic signal. As an alternative to sequence-based barcoding, we developed fluorescently labeled inter-simple sequence repeat (FISSR) primers that amplify fragments with a high level of variation at low taxonomic levels. We developed a FISSR fragment database and designed strategies for matching query samples to vouchered reference samples. In our preliminary tests this method was more accurate than sequence-based barcoding for species identification. It also had the advantage of being able to identify hybrids when parent species were included in the reference database.
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1 - Fairchild Tropical Botanic Garden, Center for Tropical Plant Conservation, 11935 Old Cutler Road, Coral Gables, Miami, Florida, 33156-4242, USA
Presentation Type: Oral Paper:Papers for Sections
Date: Monday, July 31st, 2006
Time: 4:45 PM