Systematics Section / ASPT
Murdock, Andrew G. , Mishler, Brent D. , Oliver, Melvin J. , Kuehl, Jennifer V. , Boore, Jeffrey L. , Karol, Kenneth G. , Mandoli, Dina F. , Everett, Karin .
Complete chloroplast genome sequence of the moss Tortula ruralis and structural arrangement relative to other green plant chloroplast genomes.
THE recent emergence of complete chloroplast sequencing has greatly increased our understanding of genome structure, codon usage, genome evolution, and is beginning to aid efforts in reconstruction of deep-level phylogeny of green plants. With these goals in mind, sequencing chloroplast genomes from a broad sampling across green plants is an essential first step. The moss Tortula ruralis, common in arid regions around the globe, is a model system for studying the biology of desiccation-tolerance. Chloroplasts from a wild collection of Tortula ruralis were extracted using Fluorescence Activated Cell Sorting (FACS). Rolling circle amplification (RCA) was used to amplify DNA from the isolated chloroplasts, which was subsequently subcloned and shotgun sequenced to an average of 8x coverage for the genome. A few final gaps were filled using manual PCR. Gene content of the Tortula chloroplast is comparable to the previously sequenced chloroplast genome of the moss Physcomitrella patens; as with Physcomitrella, Tortula lacks the genes ccsA and rpoA in the chloroplast which appears to be a shared derived character for mosses. However, Tortula lacks the large inversion present in the large single copy (LSC) region of Physcomitrella and the gene order is thus similar to that of the liverwort Marchantia. Genome structure and gene content is compared with other green plant chloroplast genomes and placed into a phylogenetic context.
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1 - University of California, Berkeley, Department of Integrative Biology, 1001 Valley Life Sciences Bldg. #2465, Berkeley, California, 94720-2465, USA
2 - University of Missouri, Division of Plant Sciences, 205 Curtis Hall, Columbia, Missouri, 65211, USA
3 - DOE Joint Genome Institute and Lawrence Berkeley National Lab, Evolutionary Genomics Program, 2800 Mitchell Drive, Walnut Creek, California, 94598, USA
4 - DOE Joint Genome Institute, Department of Evolutionary Genomics, 2800 Mitchell Drive, Walnut Creek, California, 94598, USA
5 - University of Washington, Department of Biology, Box 355325, Seattle, Washington, 98195-5325, USA
6 - University of Washington, Department of Biology & Center for Developmental Biology, Box 355325, Seattle, Washington, 98195, USA
Presentation Type: Poster:Posters for Sections
Location: Auditorium/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 12:30 PM