Systematics Section / ASPT
Morton, Cynthia , Rabbitt, Jodi .
Angiosperm phylogeny based on nuclear sequence information.
XANTHINE dehydrogenase belong to the molybdenum cofactor dependent hydroxylase class of enzymes. This gene is thought to play an important metabolic role in purine metabolism and have been found to be induced by drought stress. XDH gene sequences for 200 genera including several genera of gymnosperms were analyzed using parsimony (MP) and bootstrap analyses. Primers were designed for the gene by using animal and plant species. These primers were used to amplify a 1486 bp fragment. Approximately 7 indels were examined ranging from 3- 40 bps in length. The number of informative characters found are greater then has been reported for the rbcL, atpB and matK genes. Traditionally, chloroplast genomic regions have been used for phylogenetic inference in angiosperms. The XDH gene evolves much faster than the traditionally used plastid genes. This nuclear gene tree and the chloroplast gene trees reported are highly congruent and will be discussed. The robustness of the strict consensus tree supercedes all individual or multigene analyses. XDH sequences also provide good resolution within angiosperm orders such as the Ericales. A combined analysis of XDH and complementary angiosperm datasets should provide the framework to enhance resolution and internal support in angiosperm phylogenetics.
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1 - Carnegie Museum of Natural History, 4400 Forbes Avenue, Pittsburgh, Pennsylvania, 15213, USA
Presentation Type: Oral Paper:Papers for Sections
Location: 144/Performing Arts Center
Date: Tuesday, August 1st, 2006
Time: 10:45 AM