Economic Botany: Applied Plant Biology
Mathur, Meeta , Kumar, S. , Ramawat, K.G. .
In vitro production of guggulsterones in organized and unorganized tissues of Commiphora wightii.
COMMIPHORA wightii (Arnott) Bhandari has become an endangered medicinal tree (Burseraceae) because of its over-exploitation for gum resin, slow growth of plant, poor seed-set and excessive tapping. The gum resin of the Commiphora wightii tree is revered in the Indian system of medicine: Ayurveda for joint pain, hypercholestrolemia, hyperlipidemia, obesity and arthritis. The lipid lowering activity is due to presence two closely related steroidal ketones i.e. guggulsterones-E and guggulsterone-Z. Unavailability of sufficient guggul from natural resources and destruction of plant, initiated the search for alternative methods of guggulsterones production. Excellent alternative for that are somatic embryogenesis, differentiated and non-differentiated callus, cell culture and cloning. We report somatic embryos formation from zygotic explants and callus, demonstrate the development of resin canals during torpedo stage somatic embryos, guggulsterones accumulation during somatic embryogenesis, embryonic and non-embryonic callus and cell cultures. Somatic embryos and secondary somatic embryos were obtained on MS medium by reciprocal transfer between the medium containing 2, 4, 5-T and kinetin and that devoid of plant growth regulators. Embryogenic callus was grown on the medium containing BA and IBA (0.1 mg/l each). Resin canals were observed in torpedo shaped and cotyledonary stage embryo. Early stages of somatic embryogenesis were devoid of it. The non-embryonic callus cultures were established from immature zygotic embryos and leaf explants grown on MS medium containing 2, 4-D and kinetin and cell cultures were obtained from the callus grown on same liquid medium. Guggulsterone content of embryonic callus (16.5µg/g) was higher than non- embryonic callus (5.2µg/g) and cell culture (7.4µg/g). Guggulsterone-E and -Z contents of in vitro embryonic cultures were ¼ - and ½ -folds of that found in zygotic embryos, but several fold higher than callus cultures. These cultures are being exploited for the production of these bioactive molecules by biotechnological methods.
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1 - M. L. Sukhadia University, Department of Botany, Laboratory of Bio-Molecular Technology, Udaipur, 313001, India
2 - M. L. Sukhadia University, 313001, India
Presentation Type: Oral Paper:Papers for Topics
Location: 304/Bell Memorial Union
Date: Tuesday, August 1st, 2006
Time: 11:00 AM